南宁师范大学苏炜&林陈想等：具有近红外响应的卟啉自组装纳米光敏剂实现高效I型光动力和光热治疗
2025-05-22 15:09:46
作者：中国科学材料 来源：中国科学材料
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具有近红外光响应的I型光敏剂对氧的依赖性较低，可实现有效的电子转移，在缺氧微环境光动力治疗中具有极大的应用潜力。
近日，南宁师范大学苏炜教授、林陈想博士、广西中医药大学李培源研究员和北京科技大学姜建壮教授等人在Science China Materials发表研究论文，通过5,10,15,20-四(4-氨基苯基)卟啉（TAPP）与N-苄氧羰基-L-亮氨酸（Cbz-Leu）的共组装策略，成功构建了超分子纳米光敏剂TAPP@Cbz-Leu。
本文要点
1) 在近红外光（808 nm）照射下，由于TAPP与Cbz-Leu之间的非共价的紧密结合，可诱导光生电子从Cbz-Leu向三重态TAPP转移，生成TAPP·-，因 此，TAPP@Cbz-Leu可以显著生成O2·-。此外，TAPP@Cbz-Leu在808 nm近红外光照射下具有优异的光热性能。2) 体内实验表明，TAPP@Cbz-Leu对MRSA感染的动物伤口模型具有良好的抗菌效果，并能有效促进伤口愈合。
本研究首次通过简单共组装策略实现了II型光敏剂向I型光敏剂的转化，为开发新型近红外响应的光疗材料的构建提供了新思路。
Figure 1. Schematic illustration of the conversion of type II photosensitizer (TAPP) triggered by 660 nm light to type I photosensitizer (TAPP@Cbz-Leu) triggered by 808 nm NIR light.Figure 2. UV-vis absorption spectroscopy of (a) TAPP@Cbz-Leu with different TAPP and Cbz-Leu stoichiometric ratios, and (b) TAPP, Cbz-Leu, the mixed solution of TAPP and Cbz-Leu in DMSO, and TAPP@Cbz-Leu in water. (c) Fluorescence intensities of TAPP in DMSO and TAPP@Cbz-Leu in water. (d) TEM image of TAPP@Cbz-Leu. (e) Size distribution in water detected by DLS. (f) FT-IR spectra of TAPP, Cbz-Leu and TAPP@Cbz-Leu.
Figure 3. (a) ROS generation of TAPP@Cbz-Leu in water with irradiation, detected by DCFH. (b) ROS generation of TAPP, ICG and TAPP@Cbz-Leu in water with irradiation. (c) 1O2 production of TAPP@Cbz-Leu in water with irradiation detected using SOSG. (d) O2·- production of TAPP@Cbz-Leu in water with irradiation detected using DHE. (e) O2·- production of TAPP and TAPP@Cbz-Leu in water upon irradiation. (f) O2·- production of TAPP and TAPP@Cbz-Leu upon irradiation under normal and hypoxic conditions. (g) ESR spectra of O2·- trapped by DMPO in TAPP@Cbz-Leu with or without irradiation. (h) Energy levels of TAPP@Cbz-Leu and TAPP. (i) Nanosecond transient absorption spectra of TAPP and TAPP@Cbz-Leu dispersed in water (0.5 mM). All the irradiation is 808 nm, 0.5 W/cm2.
Figure 4. (a–c) Viability and (d) representative pictures of MRSA, S. aureus and E. coli following treatment with TAPP@Cbz-Leu. (e) Live/Dead staining of MRSA treated with TAPP@Cbz-Leu. (f) Detection of ROS generation of TAPP@Cbz-Leu via DCFH probe. (g) SEM images of S. aureus and E. coli with and without treatment with TAPP@Cbz-Leu. Error bars represent the mean ± SD (n ≥ 3). All the irradiation is 808 nm, 0.5 W/cm2.
Wei Su (苏炜) received his BS and PhD degrees from Shandong University, and then he worked at North Carolina State University as a postdoc. He joined Nanning Normal University in 2008 and is currently a full professor. His work focuses on porphyrin and phthalocyanine materials and their applications in photocatalysis.
Lei Cen (岑磊) received his Master’s degree under the supervision of Prof. Wei Su and Dr. Chenxiang Lin at Nanning Normal University. His research interest mainly focuses on phototherapy antibacterial of porphyrin and phthalocyanine self-assembled nanomaterials.
Dong Yang (杨栋) is currently studying for a Master’s degree under the supervision of Prof. Wei Su and Dr. Chenxiang Lin at Nanning Normal University. His research focuses on preparation of functional hydrogels and antimicrobial phototherapy.
Chenxiang Lin (林陈想) received her BS degree from Guangxi Normal University in 2015 and PhD degree from University of Science and Technology Beijing in 2021. Then, she worked at Guangxi Key Laboratory of Natural Polymer Chemistry and Physics of Nanning Normal University as a lecturer. Her research focuses on the design and synthesis of porphyrin and phthalocyanine functional materials for phototherapy antibacterial and photocatalytic hydrogen production.
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